My application requires read lengths of 200-250bp, which looks doable with the 100bp and 150bp paired end reads on HiSeq and MiSeq. I was wondering
1) Is there another technology currently in the market with read lengths in 200-250bp region? I've tried the PGM 200bp kit which performed abysmally. Most reads had at least 1 read error, which is not suitable for my application.
2) If I use the illumina platform, how many # reads can I expect for the HiSeq and MiSeq? With paired end 150bp reads, what is the max length of the fragment with adapters?
1) Is there another technology currently in the market with read lengths in 200-250bp region? I've tried the PGM 200bp kit which performed abysmally. Most reads had at least 1 read error, which is not suitable for my application.
2) If I use the illumina platform, how many # reads can I expect for the HiSeq and MiSeq? With paired end 150bp reads, what is the max length of the fragment with adapters?
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