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Old 01-18-2017, 06:37 AM   #3
HelenaSC
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Location: Spain

Join Date: Jun 2013
Posts: 17
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Hi Joni,

Yes, the larger fragments are supposed to be overamplification. I made a mistake using the recommendation from the tables, i choosed the wrong conditions regarding the number of cycles in relation to tje number of primers from our panel... Anyway, I repeated the protocol with the correct conditions and, altough some of the samples resulted in much better libraries profile, others just remained the same...

We tried to Run these samples together on a Run and we are experiencing some problems, it seems that the MiSeq was able to detect the clusters (1400) but it wasn't able to give us any more results, and none of the clusters has good passing filter QC...strange... Here is an image from the BySpace, maybe it is more easy to understand...Did you performed a Run?

Imagen1.jpg

Imagen2.jpg
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