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Old 06-20-2016, 08:24 AM   #3
thermophile
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Location: CT

Join Date: Apr 2015
Posts: 243
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I've run truseq lt with my homebrew dual indexed amplicons. It works fine but I'm pooling very different things and expect to have some wildly different pooling effeciencies. For the sample sheet you'll need to add NN to the reverse index, and NNNNNNNN for the forward (of course after checking that none of your nextera indexes start with the 6pb of the truseq index)
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