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Old 12-21-2017, 10:27 AM   #1
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Location: Arkansas

Join Date: Dec 2017
Posts: 5
Default ddRAD Post-PCR Bioanalyzer Has Multiple Peaks


I have a problem with a post-PCR ddRAD library that was prepared using Peterson (2012). It was prepared along with another index for sequencing on one Illumina lane, and the pre-PCR size selection for both indices was supposed to be 454-509 bp.

However, the Bioanalyzer results show two smaller peaks for one of the indices that shouldn't be there (see attached images), and the smaller peaks should be outside the size-selection range. Thus, it must have post size selection.

Does anyone know what might have caused the two smaller peaks to occur, and should I worry about it during sequencing?

Thank you,

Attached Images
File Type: jpg L1712-02-I8.jpg (76.4 KB, 16 views)
File Type: jpg L1712-02-I9.jpg (81.5 KB, 17 views)
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