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Old 12-21-2017, 01:57 PM   #4
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,189

If you have run size selected tags on BA or still have remaining to run you can check for the presence of those peaks. I think the size selection has been sub optimal.

Other weak possibility is over amplification. I wonder what was the PCR input and final library elution volumes.

If it turns out to be over-amplification then a new library needs to be prepped. If it is result of size selection then you just will loose some reads and library can be sequenced.
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