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Old 12-21-2017, 02:33 PM   #6
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,196
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So you have used in total 20 ng of size selected tags for PCRs and got 45.6 ng/ul after pooled PCR clean up. I am interested to know what was the elution volume from beads to look at possibility of over-amplification.
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