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Old 05-10-2011, 05:55 AM   #21
steven
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Location: Southern France

Join Date: Aug 2009
Posts: 269
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Quote:
Originally Posted by censinis View Post
Hi guys,

I am particularly interested on directional RNA-seq to be determined by means of RNA-seq and Illumina HiSeq 2000. Same authors (like N.Croucher of Sanger) already mentioned few approaches but I am wondering if anyone already tested them with bacterial totRNA. In particular I am looking at protocols suggested for ribosomal RNA depletion, RNA fragmentation and retro-transcription. Could you be so gentle to help me?

Thanks in advance
Best
SC
About the DSN normalization:
- a paper
- a guide
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