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Old 04-07-2010, 09:16 AM   #1
chrisbala
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Location: North Carolina

Join Date: Jan 2010
Posts: 82
Default converting tophat .wig to bigwig for upload into ucsc

Hi,

I'm trying to get my wig file output from tophat into ucsc.

in the past i've had no trouble because my files were small enough. now i am trying to convert into bigwig using UCSCs tools (bedGraphtoBigWig). Unfortunately, I'm getting an error when I do this:

line 10544021 of coverage_familiar_new.wig: start and end coordinates the same
They need to be at least one apart

Is this a problem with how tophat is writing the file? or a problem with the conversion utility? or a problem with me?

thanks!

Chris
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