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Old 06-23-2017, 11:47 AM   #3
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 243

The 515F/806R primers that are out there (Caparosso or Kozich primers) are designed to be biased against chloroplast and mitochondria. My tricks for tissues are use a ton of DNA because most of what you are measuring is host, also spike in a tiny amount of non-illumina/non-barcoded 515f/806r
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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