SEQanswers - View Single Post - 16S sequencing tissue

adam.geber · 08-15-2017, 10:00 AM

Hi thermophile,

Can you clarify the purpose of spiking in the non-barcoded primers to minimize host mitochondrial contamination? Wouldn't you expect these to bind equally well to the bacterial V4 region?

08-15-2017, 10:00 AM	#4
adam.geber Member Location: United States Join Date: May 2014 Posts: 40	Hi thermophile, Can you clarify the purpose of spiking in the non-barcoded primers to minimize host mitochondrial contamination? Wouldn't you expect these to bind equally well to the bacterial V4 region?