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Old 07-17-2018, 01:46 AM   #5
Pauline dlp
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Location: Philippines

Join Date: Mar 2018
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It seems you are right. The adapters are modified to have a phosphate group on the 5' and phosphorothioate on the 3' end before the terminal T to protect it from 3' -> 5' exonuclease activity.

I am not sure about the Nextera Exome adapters but this article describes the structure of adapters for Truseq and Nextera:
http://bioinformatics.cvr.ac.uk/blog...mer-sequences/
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