SEQanswers - View Single Post - What is the longest fragment for Illumina Mate pair end seq library?

monad · 05-18-2011, 01:38 PM

Make sure that your starting input DNA is enough. Longer the insert, you will need a lot more input DNA. We are talking about ~10ug for end repair for ~12kb insert mate pair, so starting material is whatever the recovery rate after shearing.

05-18-2011, 01:38 PM	#3
monad Member Location: California Join Date: May 2008 Posts: 40	Make sure that your starting input DNA is enough. Longer the insert, you will need a lot more input DNA. We are talking about ~10ug for end repair for ~12kb insert mate pair, so starting material is whatever the recovery rate after shearing.