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Old 06-10-2011, 06:59 AM   #3
Location: Boston

Join Date: Sep 2010
Posts: 14

I'm doing the same thing and have been able to increase my yield using phenol/chloroform extraction with phase-lock gels to clean up my ChIPped DNA after reverse cross-linking. Also, you might want to try quantitating your DNA with a flourescence-based ds DNA kit (I like Invitrogen Quantit-IT) because your sample concentration might be below the detection limit of the nanodrop.
kalidaemon is offline   Reply With Quote