SEQanswers - View Single Post - ChipSeq library construction from tissue and DNA purity issues

kalidaemon · 06-10-2011, 06:59 AM

I'm doing the same thing and have been able to increase my yield using phenol/chloroform extraction with phase-lock gels to clean up my ChIPped DNA after reverse cross-linking. Also, you might want to try quantitating your DNA with a flourescence-based ds DNA kit (I like Invitrogen Quantit-IT) because your sample concentration might be below the detection limit of the nanodrop.

06-10-2011, 06:59 AM	#3
kalidaemon Member Location: Boston Join Date: Sep 2010 Posts: 14	I'm doing the same thing and have been able to increase my yield using phenol/chloroform extraction with phase-lock gels to clean up my ChIPped DNA after reverse cross-linking. Also, you might want to try quantitating your DNA with a flourescence-based ds DNA kit (I like Invitrogen Quantit-IT) because your sample concentration might be below the detection limit of the nanodrop.