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Old 07-13-2011, 11:07 AM   #5
rgejman
Junior Member
 
Location: New York

Join Date: Nov 2010
Posts: 4
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Does this "double priming" have to happen once or every time you load a v1 vs. v3 flow cell? I actually buy 200 cycle kit (actually 202 cycle kits) and aliquot them into 4x 50 cycle kits (for which I can actually run 51 cycles—works like a charm). If double priming happens every time I switch then this will not be feasible...
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