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Old 07-26-2011, 12:15 AM   #4
Liting
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Location: China

Join Date: Jul 2011
Posts: 12
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Hello,Monad.I have some problems and hope for your help.Thank you.I used 20ug DNA for end repair for 6kb insert mate pair.The protocal is :the DNA with cycling adaptors,size selection after nebulization,circularization & linear Digestion and nebulized again,then library immobilization for paired-end library construction.I used agarose gel electrophoresis to check the size of library. It's bigger than 100bp.The result is abnormal.I can't figure out why.Please give me some advices.Thank you very much!
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