View Single Post
Old 09-15-2011, 12:47 PM   #5
james hadfield
Moderator
Cambridge, UK
Community Forum
 
Location: Cambridge, UK

Join Date: Feb 2008
Posts: 221
Default

you could add a 4bp random sequence in your barcode read or at the 5'end of your oligo for ligation. This way you can see if a read is a duplicate of PCR. You should not see the same random sequence, unless PCR has amplified it so.
james hadfield is offline   Reply With Quote