SEQanswers - View Single Post - How to differentiate between PCR duplicates and real data?

james hadfield · 09-15-2011, 12:47 PM

you could add a 4bp random sequence in your barcode read or at the 5'end of your oligo for ligation. This way you can see if a read is a duplicate of PCR. You should not see the same random sequence, unless PCR has amplified it so.

09-15-2011, 12:47 PM	#5
james hadfield Moderator Cambridge, UK Community Forum Location: Cambridge, UK Join Date: Feb 2008 Posts: 221	you could add a 4bp random sequence in your barcode read or at the 5'end of your oligo for ligation. This way you can see if a read is a duplicate of PCR. You should not see the same random sequence, unless PCR has amplified it so.