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Old 10-31-2013, 04:14 AM   #1
Senior Member
Location: Norway

Join Date: Aug 2013
Posts: 266
Default An ide for RNAseq validation with qPCR

Just a thought:

1. Make list of all sign. changed genes called from Cuffdiff, edgeR and DESeq. This will be high sensitive I guess, but low specificity.

2. Correlate against clinical characteristica.

3. Validate all genes which sign. correlate with clin. char. with qPCR

Is this totally of?
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