View Single Post
Old 09-24-2014, 07:19 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
Default

Quote:
Part 2, as I understand, is the "left side" selection. The addition of 10uL of AMPure beads to 75uL supernatant is a 10/75 = 0.13x bead ratio; would this not lead to a almost-complete loss of material? It seems to me that a more appropriate AMPure bead volume would be 60uL (60/75=0.8) for a 0.8x bead ratio. Perhaps I'm missing something.
Supernatant from Part 1 contains 0.6x bead solution, so by addition of bead in Part 2 it will be 0.8x.
nucacidhunter is offline   Reply With Quote