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Old 05-18-2010, 10:23 PM   #1
seattle
Junior Member
 
Location: Shanghai

Join Date: May 2010
Posts: 2
Smile Hi, everybody, Do you agree amplification will delivery bias to RNA-seq

I am currently working with Affymetrix platform. We are evaluating the reproducibility of microarray experiments, finished pilot, training and test studies, with large cohort of samples. It seems to me that microarray technology requires high quality RNA, and RNA amplification, which always delivery bias.

On the other hand, I am very interested to receive quick messages from SEQanswers: whether RNA-seq technology provided by next generation sequencing platforms also demands high quality RNA together with RNA amplification. It could delivery some question to this platform.

What do you think?
Best wishes,
Xun

Last edited by seattle; 05-18-2010 at 10:25 PM.
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