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Old 05-19-2010, 03:35 AM   #4
seattle
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Location: Shanghai

Join Date: May 2010
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Quote:
Originally Posted by seqgirl123 View Post
This site is useful as well for a collection of library protocols from Illumina: http://keck.med.yale.edu/microarrays...protocols.html
It gives pdf library protocols for the following:
Preparing Samples for Whole Transcriptome Profiling

Many thanks to ECO for creating and maintaining the forum. I do appreciate your attached link, and read the paper with great interest.

Illumina RNA-seq requires RNA with RIN value higher than 8. It seems to me that, to perform RNA-seq, it is doable to use samples from Clontech. But it is really challenging to apply this platform to identify predictors using real clinical samples, like tumor biopsy.
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