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Old 01-17-2018, 11:44 PM   #7
liaoyunshi
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Location: Hong Kong

Join Date: Jul 2017
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Quote:
Originally Posted by finswimmer View Post
Hello,



yes, because the PEG concentration in the solution is the critical reagence in the size selection process. I've found a nice blog post about it some time ago here: http://core-genomics.blogspot.de/201...eads-work.html

fin swimmer
Hi,

I also found it! Thanks.

Actually before reading this post, I just wondered why the second selection with less bead amount can capture small DNA. And I finally realize that it is the bead solution makes it capture different size DNA, and the bead solution is more and more concentrated after beads pouring in for the second selection.
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