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Old 10-07-2010, 03:54 AM   #1
strob
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Location: Belgium

Join Date: Nov 2008
Posts: 79
Default low 454 coverage combined with high solexa coverage

Hi,

has anybody experience with combining following two datasets:

1X coverage of 454 reads (backbone)
30X coverage of solexa reads

background: we are talking about a non sequenced plant genome. So I would use the 1x 454 reads as a backbone for the solexa reads to perform a de novo assembly.

Question: is a 1X 454 coverage in this case a waste of money or a real help in the assembly? Somebody experience with this?
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