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Old 06-28-2011, 06:35 AM   #1
ETHANol
Senior Member
 
Location: Western Australia

Join Date: Feb 2010
Posts: 308
Default TruSeq compatible PCR primers

I'm doing ChIP-seq and would like to start using the TruSeq Adaptors for barcoding (single read). I have the sequences of the TruSeq adaptors from Illumina but they do not give out the sequences of the PCR primers.

Has anyone made their own PCR primers that work with the TruSeq adaptors?

If it's not illegal to post this trade secret, does anyone know the sequence of the Illumina PCR primers for TruSeq?
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