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Old 07-06-2011, 06:25 AM   #6
ETHANol
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Location: Western Australia

Join Date: Feb 2010
Posts: 308
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Thanks. I put the order in. We'll see if it works.

The phosphorotiolation is suppose to increase efficiency with proofreading polymerases. Maybe it's not necessary but it couldn't hurt.
http://nar.oxfordjournals.org/conten.../3551.abstract

I also Googled my primer sequences and found this:
http://openwetware.org/images/1/10/Geller_exome.pdf

So it seems like it should work.
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