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Old 08-29-2011, 07:20 AM   #1
Location: Tokyo, Japan

Join Date: May 2010
Posts: 27
Default microRNA normalization


Does anyone know the reasonable normalization methods for microRNA data analysis?
I already trimmed adaptor, mapped these reads to the reference, and counted the number of reads that mapped on the known microRNA seq.

What I have is a list of known mature microRNAs' ID and sequence, and the number of reads mapped to this miRNAs.
I have 5 samples and want to compare differential expression among them.

How do you normalize the number of mapped reads in order to compare the results from different samples?
Any RPKM-like methods?

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