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Old 09-25-2012, 07:14 PM   #1
Elsie
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Location: Australia

Join Date: Mar 2011
Posts: 85
Default Agilent Methyl-Seq - enrichment + BS + sequencing

Dear all, long time listener first time caller here.
I've got some Agilent Methyl-Seq data, I'm looking for feedback from anyone with experience with this data - i.e. enrichment for regions of interest, then BS then sequencing. I've been using Bismark (great!, thanks very much Babraham!) and have just given Trim Galore! a whirl. Any tips on parameters used, trimming etc, I've only got about 66% mapping efficiency and would like to improve upon that.
thanks.
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