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Old 03-04-2013, 05:40 PM   #1
Location: Canada

Join Date: Sep 2012
Posts: 21
Default DNA-seq and Read Trimming: Basic Question

Hi Everyone:

I am doing exome sequencing and I just had a basic question about read trimming. Is read trimming at the 3' end AND/OR by base quality score completely necessary? What influence will NOT trimming have on downstream variant calling? I was thinking that low quality bases would be accounted for by their low base qualities and thus we would not have to completely omit or "trim" these bases?


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