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Old 01-26-2011, 01:43 AM   #2
Senior Member
Location: Cambridge, UK

Join Date: Sep 2009
Posts: 625

Hi michy,

The command you used looks ok-ish, but

(a) -q is the default, however Bowtie will then assume Phred33 qualities. The HiSeq seems to chuck out Phred64 encoded data though, try specifiying -q --phred64-quals
(b) -I 0 is not not required as it is the default
(c) If you still don't get any alignments you might want to increase -X to a higher value, maybe your insert sizes are just larger than 300bp?

Hope (a) or (c) will fix it.
fkrueger is offline   Reply With Quote