SEQanswers - View Single Post - Bowtie and reads that failed to align: (100.00%)

michy · 01-26-2011, 02:12 AM

Hello fkrueger,
thank you for your reply. Your suggestions esp. a) and c) they did improve the alignment by 15% which means 85% still failed to align. However, I was testing the command on a small subset of the data. I will try some more reads and get back to you.

Thank you again and any other suggestions welcome.

Michy

01-26-2011, 02:12 AM	#3
michy Junior Member Location: oxford Join Date: Aug 2008 Posts: 5	Hello fkrueger, thank you for your reply. Your suggestions esp. a) and c) they did improve the alignment by 15% which means 85% still failed to align. However, I was testing the command on a small subset of the data. I will try some more reads and get back to you. Thank you again and any other suggestions welcome. Michy