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Old 02-13-2015, 07:26 AM   #10
Location: Sweden

Join Date: Oct 2014
Posts: 11

Dear Brian,

Thanks for the extensive response and clarification on how the program works. Very much appreceated.

I kind of forgot to mention that I am using it on RNA-seq data from a HiSeq 2500. I currently don't have access to the mapped file, but I'll try it on them as soon as I can.

The thing is I am using the program (right now at least) just to determine if I am sequencing deep enough or if I can multiplex furthere. I don't need a perfect curve, just an estimate. Was thinking maybe to trim and then run, but shouldn't gain much from that...


PS. Was also thinking that the spikes are nice in a way as a quility indiciation. I have instances of much higher spikes in my data.
arash82 is offline   Reply With Quote