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Old 04-25-2017, 05:50 PM   #18
Location: New Zealand

Join Date: Jul 2014
Posts: 20

Great, thanks. How did you estimate the average coverage from the % uniqueness? I know I can do it more accurately with BBNorm (which says read depth median at 55x) but I'm curious how you did it from that plot. These are 100 b reads, but it's about 50 Gb of sequencing from a genome we are expecting to be ~600 Mbp, so you are not far off if coverage were even.
TomHarrop is offline   Reply With Quote