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  • shrujan
    Member
    • Aug 2012
    • 38
    #1

    RNA Sequencing QC Error while using with Sequence_QC.sh file

    Hi,

    This is Shrujan kumar Madadha, I had an error while running QC for Drosophila Yukuba fastq RNA file using Sequence_QC.sh file of FASTX Toolkit. So please guide me to resolve the follwing error:

    shrujan@ubuntu:~/NGS_Practice/RNA$ sh Sequence_QC.sh SRR530661_1.fastq -o DY_RNA_QC
    awk: program limit exceeded: maximum number of fields size=32767
    FILENAME="SRR530661_1.tab" FNR=1 NR=1
    Sequence_QC.sh: 30: Sequence_QC.sh: xgawk: not found
    Segmentation fault (core dumped)

    I couldn't understand why it is stopped. Can anyone please guide me with the perfect solution.

    Thank you and i hope a quick reply.
    Tags: fastx toolkit, quality control, rna sequencing
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