[odile]

Hi, thanks for your response. The problem is that I need to clean up the extract before I attach the adaptors (after the blunt end and adenylation steps). Do you know any other method that would retain the fragments <70bp?
I was wondering if maybe people who work with RNA would have an idea?

[JUdw]

Hi, thanks for your response. The problem is that I need to clean up the extract before I attach the adaptors (after the blunt end and adenylation steps). Do you know any other method that would retain the fragments <70bp?

End-repair, A-tailing and adpter ligation is combined in the Nextera sample prep protocol:

http://www.epibio.com/item.asp?ID=566

[odile]

I had writen to Nextera a few months ago because I wanted to use their kit without the shearing part and here is what they wrote back:
"Unfortunately, 100 bp DNA fragments are too small for use with Nextera technology. The best products we could tell you to use would be the End-IT End repair Kit to generate blunt ends on the DNA fragments, followed by ligation of the adapters to the fragments with our Fast-Link DNA Ligase."