Tweet
has anyone used any other method for extracting dna samples from liquid environmental samples other than filters ?
how about soil?
how about soil?
-
-
What are you interested in looking at? Bacteria, eukaryotes, viruses, total? There are other methods for concentrating cells (differential centrifugation, tangential flow etc.) but it depends on what you're looking for and the volumes you're dealing with.
With soil there are a million different extraction procedures in the literature, many good and bad, but need a bit more detail to help
-
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
Comment