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  • bzguy
    Junior Member
    • May 2011
    • 4

    #1

    featureCounts - does it normalize by gene length?

    Hello,
    I'm starting to analyze RNA seq data, and I was hoping you can please help me clear something up

    I am using FASTQ groomer, then TopHat2 to align, and then featureCounts, to get a number of reads per gene.

    Is the numbers of reads per gene (output) of featureCounts normalized to the gene length?
    If not, how can I do that? I want to end up with number of reads per gene, regardless of isoforms etc.

    Thanks
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    featureCounts is going to count reads per feature ("-t exon") that you specify and then will summarize the counts per gene ("-g gene_id"). It is going to report raw read counts and does not normalize the data.

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