Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • fadista
    Member
    • Sep 2008
    • 37

    multiplex few variants in hundreds of samples

    Dear all,

    I would like to know if someone is working (or knows someone that is working) on a method to be able to multiplex sequencing of very few variants (let say 100 SNPs) on hundreds or maybe thousands of samples in a single HiSeq run. There is a market need for this kind of product but as far as I know, no solutions are yet available.
  • Heisman
    Senior Member
    • Dec 2010
    • 534

    #2
    Would this be appropriate? http://www.illumina.com/technology/g...ing_assay.ilmn

    Comment

    • Bukowski
      Senior Member
      • Jan 2010
      • 388

      #3
      Originally posted by fadista View Post
      Dear all,

      I would like to know if someone is working (or knows someone that is working) on a method to be able to multiplex sequencing of very few variants (let say 100 SNPs) on hundreds or maybe thousands of samples in a single HiSeq run. There is a market need for this kind of product but as far as I know, no solutions are yet available.
      Already answered elsewhere on the forums:

      We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of s …

      Comment

      Latest Articles

      Collapse

      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        07-01-2026, 11:43 AM
      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 07-02-2026, 11:08 AM
      0 responses
      10 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-30-2026, 05:37 AM
      0 responses
      13 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-26-2026, 11:10 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-17-2026, 06:09 AM
      0 responses
      54 views
      0 reactions
      Last Post SEQadmin2  
      Working...