scaffold and contig

[chengeng]

Hi all,
Does somebody know what's scaffold and the differences between scaffold and contigs? Thank you very much.

Geng Chen

[spenthil]

A contig is a contiguous length of genomic sequence.
A scaffold is composed of contigs and gaps. Gap length can be guessed by incorporating information from paired ends or mate pairs (difference between the two).

[chengeng]

Thank you very much. I understand it now.

[flxlex]

This has been very helpful. I'd recommend the site to my Dad. he's into this kind of biz. Thank you so much for sharing this with us. More power to you and to your site!

Scaffold <---this was a link to a store that sells construction scaffolding

As a moderator, I should perhaps remove this post as it is off-topic and kind of spam. But, I won't, it is too funny...

[gsgs]

at genbank's mallard genome ftp, they give an ~1.1GB fasta
file of "unplaced scaffolds" with 78487 sequences
of length 100-5998093
When I try to match them to the chicken chromosome 1
then I get a continuous distribution in matching subsequences.
Shouldn't there be a sharp peak for those mallard "scaffolds"
that are from chromosome 1 , shouldn't they be detectable this way ?

15% of chicken and turkey 28-subsequences
in chromosome 1 do match
only ~3% in chicken vs.human or chr1 vs. chr2

chromosomes are not available for mallards at genbank

ftp://ftp.ncbi.nih.gov/genbank/genom...affolds/FASTA/

------------edit-------------------------------
I found:
> An in silico digest of the chicken genome, which is very similar [25,26], predicts similar genome
> fractions of the RRLs of 4.1% for AluI, but only 0.2% for HhaI (data not shown).

don't know what that means

25. Fillon V, Vignoles M, Crooijmans RPMA, Groenen MAM, Zoorob R, Vignal A: FISH mapping
of 57 BAC clones reveals strong conservation of synteny between Galliformes and
Anseriformes. Anim Genet 2007, 38:303-307. PubMed Abstract | Publisher Full Text

26. Skinner BM, Robertson LBW, Tempest HG, Langley EJ, Ioannou D, Fowler KE,
Crooijmans RPMA, Hall AD, Griffin DK, Völker M: Comparative genomics in chicken and
Pekin duck using FISH mapping and microarray analysis.
BMC Genomics 2009, 10:357. PubMed Abstract | BioMed Central Full Text | PubMed Central
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[syfo]

As a moderator, I should perhaps remove this post as it is off-topic and kind of spam. But, I won't, it is too funny...

Funny indeed
I am looking forward to such a spam in the thread about deletion, inversion and insertion...