PacBio RS II Library Prep Input

[JoeChris38]

Hi all,

I'm looking to sequence low quantities of PCR amplicon using the PacBio RS II system. I am trying to detect single nucleotide variants within these amplicons so need to minimise the number of PCR cycles I do (to avoid PCR-induced error).

So I am looking to see if anyone knows what the input DNA template concentration requirement is for the library preparation kit 2.0 (250bp-->3kb)?

Thanks,

Joe

[pacbio]

http://www.smrtcommunity.com/SampleNet/Sample-Prep

there are a number of different protocols available for download.

Roughly, 250bp requires 250ng.