Hi,
Does anyone have any experience using Bowtie2 with Tophat?
If so could you send me your tophat script?
Does anyone have any experience using Bowtie2 with Tophat?
If so could you send me your tophat script?
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Never mind. I figured it out. -
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I posted that as i got it running.. It eventually core dumped and I spoke with the designers of tophat and they said it will take serious conversions, since bwt2 uses only sam output files instead of the bwtout as before and the -un command no longer exists and the unaligned reads are in the bam file. I decided I was better off waiting for them to release an update.Comment
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Did they say they will make an update of Tophat using Bowtie2?
It would be great, so long as the Tophat output can provide accurate Mapping Quality (MAPQ field in SAM format), which Bowtie2 does...Comment
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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