Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • narges
    Member
    • Aug 2012
    • 29

    #1

    RNA-seq sample clustering using NMF package

    I want to cluster RNA-seq samples (417 samples) using based on the expression levels of a group of selected genes using NMF method. Having a matrix (mat) of expression levels with sample names as columns and gene names as rows I tried following command from Bioconductor NMF package:

    Code:
    library(NMF)
    Code:
    res <- nmf(mat, 2:10, nrun = 200, seed = 123456)
    I figure out that rank=4 is the optimum.
    First do you think it is a reasonable way of sample clustering?
    If so, should I normalize/transform the expression levels before clustering (like TMM normalization or log or asinh transformations)?
    And finally, I need to know the names of samples in each calculated clusters. Using command "basisnames" I got NULL. What command should I try to get the samples orders in clusters?

    Thanks for the help.

Latest Articles

Collapse

  • SEQadmin2
    How Immunogenomics Decodes Immunity’s Genetic Blueprint
    by SEQadmin2




    The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.

    This convergence of genetics, immunology, and computation...
    Yesterday, 05:41 AM
  • SEQadmin2
    Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
    by SEQadmin2



    CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

    Despite this, “CRISPR helped turn genome editing from a specialized technique into
    ...
    07-31-2026, 11:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 08-24-2026, 10:32 AM
0 responses
44 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 08-20-2026, 11:17 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 08-18-2026, 10:05 AM
0 responses
55 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 08-13-2026, 12:22 PM
0 responses
50 views
0 reactions
Last Post SEQadmin2  
Working...