Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • Gorbenzer
    Member
    • Apr 2012
    • 45
    #1

    Alternative (cheaper) reagents for Ion Torrent PGM sequencer

    Hi everyone,
    in my lab we are trying to find a way for reduce the running costs of our new IonTorrent PGM sequencer... any suggestion?

    Anyone found cheaper reagents that can substitute the ones sold by Life Technology?
    Tags: None
  • jimmyinfrance
    Member
    • May 2011
    • 16
    #2
    We have checked out NEB kits, work OK (although still more expensive than I'd like).

    Comment

    • Gorbenzer
      Member
      • Apr 2012
      • 45
      #3
      Originally posted by genseq
      Cheap barcoding:
      Looks really interesting, time to use google translator
      Last edited by Gorbenzer; 06-05-2012, 11:29 PM.

      Comment

      • diagen
        Member
        • Jun 2012
        • 13
        #4
        Does anybody know the sequences of primers, adaptors and immobilized oligos?

        Comment

        • HeidiJTP
          Member
          • Nov 2011
          • 22
          #5
          genseq - thanks for the latest info!

          How about the NEXTflex kits? Anyone had experience with their DNA-Seq kits or the barcoding?
          http://www.biooscientific.com/DetailProduct.aspx?itemId=459&CName=Next-Generation%20Sequencing&SubName=Ion%20Torrent-Compatible&Name=DNA%20Sequencing

          Comment

          • prooney
            Junior Member
            • Oct 2012
            • 2
            #6
            Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...

            Comment

            • salyamoff
              Junior Member
              • Sep 2013
              • 4
              #7
              Originally posted by prooney View Post
              Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...
              This is intresting question. Nobody ever tried?

              I did it with the cancer hotspot panel. Sequencing results are similar.
              Last edited by salyamoff; 04-03-2014, 11:23 AM.

              Comment

              Previous template Next

              Latest Articles

              Collapse
              • SEQadmin2
                Nine Things a Sample Prep Scientist Thinks About Before Sequencing
                by SEQadmin2


                I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

                Here are nine questions we think about, in roughly the order they matter, before...
                06-18-2026, 07:11 AM
              • SEQadmin2
                From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
                by SEQadmin2


                Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.

                The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
                ...
                06-02-2026, 10:05 AM
              View All

              ad_right_rmr

              Collapse

              News

              Collapse
              Topics Statistics Last Post
              Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population by SEQadmin2
              Started by SEQadmin2, 06-17-2026, 06:09 AM
              0 responses
              37 views
              0 reactions
              		 Last Post SEQadmin2
              by SEQadmin2
              06-17-2026, 06:09 AM  
              Sequencing the Two-Toed Sloth Genome Reveals Jumping Genes Tied to Its Extreme Metabolism by SEQadmin2
              Started by SEQadmin2, 06-09-2026, 11:58 AM
              0 responses
              100 views
              0 reactions
              		 Last Post SEQadmin2
              by SEQadmin2
              06-09-2026, 11:58 AM  
              A New Method Makes Hantavirus Genome Analysis Faster and More Accessible by SEQadmin2
              Started by SEQadmin2, 06-05-2026, 10:09 AM
              0 responses
              121 views
              0 reactions
              		 Last Post SEQadmin2
              by SEQadmin2
              06-05-2026, 10:09 AM  
              A New Single-Cell Method Maps DNA-Protein Interactions by SEQadmin2
              Started by SEQadmin2, 06-04-2026, 08:59 AM
              0 responses
              113 views
              0 reactions
              		 Last Post SEQadmin2
              by SEQadmin2
              06-04-2026, 08:59 AM  
              View All
              Working...