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  • abh
    Member
    • Aug 2012
    • 13

    novoalign mapping

    Hi,
    I want to use novoalign to map reads - allowing up to 15 mismatches for 100 bp paired-end reads

    I am new to novoalign(went through the manual) and confused with some of the options like -t,-x,-r etc..

    so for 15 mismatches i should give something like

    -t 500

    what would be -x then?

    this is the command line i am thinking to run

    novoalign -d genome -f A_1.fq A_2.fq -t 500 -r All -o SAM > out.sam

    Is this correct?


    thank you

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