Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • htchu.taiwan
    Junior Member
    • Dec 2011
    • 5

    EBARDenovo - A new RNA-seq do novo assembler for paired-end Illumina data

    Hi,

    I wrote a program for de novo assembly of paired-end RNA-seq data.

    It's a 64-bits Windows command with .Net framework http://msdn.microsoft.com/en-us/library/w0x726c2.aspx.

    You can try it on a Windows-7 PC with 16G RAM for the assembly of single run Illumina transcriptomic data (20G, 90bp~100bp paired-end reads).

    EBARDenovo is designed for finding lower-expressed transcripts even their coverage depths are very low (even less than 2x).

    The download link:
    Download EBARDenovo for free. Highly-accurate de novo assembler of paired-end RNA-Seq. A highly-accurate search-based de novo assembler of paired-end RNA-Seq for advance transcriptomic study.


    I wish you can obtained fine results by my EBARDenovo program.

    Best regards,

    I am Hsueh-Ting Chu from Taiwan. [email protected]
  • haojam
    Member
    • Jun 2010
    • 12

    #2
    Dear Hsueh-Ting Chu from Taiwan

    Good to know about your EBARDenovo. Does it support for transcriptome data generated from other platforms like ion torrent other than Illumina.

    Rocky

    Comment

    • manoharan1988
      Junior Member
      • May 2013
      • 1

      #3
      Hi Hsueh-Ting Chu,

      Could you please give me some Idea on working with denovo bacterial transcriptome data to find the expression level.

      Regards,
      Manoharan

      Comment

      Latest Articles

      Collapse

      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        Yesterday, 11:43 AM
      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM
      • SEQadmin2
        From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
        by SEQadmin2


        Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


        The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
        ...
        06-02-2026, 10:05 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 06-30-2026, 05:37 AM
      0 responses
      11 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-26-2026, 11:10 AM
      0 responses
      18 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-17-2026, 06:09 AM
      0 responses
      52 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-09-2026, 11:58 AM
      0 responses
      111 views
      0 reactions
      Last Post SEQadmin2  
      Working...