Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Fred13
    Junior Member
    • Nov 2009
    • 9

    SAMTools alignment viewer

    Hi everybody ...

    We have sequence fragments of a mutant nematode that we sent to sequenced. We used MAQGene to link fragments genome to the reference sequence of C. elegans. Then we used maq2sam-long to convert the .map file into a .sam file. With SAMTools I can visualize the alignment with tview. There is on the top the reference sequence and down all the fragments with overlap if present. I would like to know what kind of alignment browser allows sam (or bam) file. I try to use Integrative Genomics Viewer (IGV) but I can't see the aligment as in tview : the reference sequence up and all the other sequences down. Or if there is a possibility to generate a text file with the alignment of tview ?

    Thanks to the person reading this and sorry if the question is stupid ....

Latest Articles

Collapse

  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
41 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-09-2026, 11:58 AM
0 responses
102 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-05-2026, 10:09 AM
0 responses
123 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-04-2026, 08:59 AM
0 responses
114 views
0 reactions
Last Post SEQadmin2  
Working...