Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • MattB
    Member
    • Aug 2008
    • 35
    #1

    Genomic enrichment on non-model organisms...

    Hi all,

    Just wondering if anyone could give some advice about the following. We would like to enrich for fosmid end sequences in a fish (nonmodel) and shrimp species, for subsequent high coverage sequencing (454 or SOLiD) in a number of individuals. My understanding is that Nimblegen arrays are restricted to human and mouse at the moment, but Agilent technology is more flexible. Can anyone suggest what would be the most suitable technology for this type of enrichment...if it is indeed feasible?

    Thanks,
    Matt
    Tags: None
  • sigusn
    Member
    • Aug 2008
    • 19
    #2
    Hi Matt
    You can order custom sequence capture arrays from Nimblegen for any species and do the enrichment yourself, the service they provide is only for human/mouse. I have just tested it and it works great with Solexa. The arrays cost about 600$ and you order minimum of 5. The expensive thing is that you need to have the MAUI hybridization station to perform the hybridization.
    //SS

    Comment

    • MattB
      Member
      • Aug 2008
      • 35
      #3
      Hi sigusn,

      Thanks for the quick reply! Good to hear Nimblegen can provide custom capture arrays, I guess I glossed over their website a bit too fast...

      Matt

      Comment

      Previous template Next

      Latest Articles

      Collapse
      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        Today, 11:43 AM
      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM
      • SEQadmin2
        From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
        by SEQadmin2


        Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.

        The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
        ...
        06-02-2026, 10:05 AM
      View All

      ad_right_rmr

      Collapse

      News

      Collapse
      Topics Statistics Last Post
      New AI Model Captures Long-Range Genomic Signals to Improve RNA Splice Site Prediction by SEQadmin2
      Started by SEQadmin2, Yesterday, 05:37 AM
      0 responses
      8 views
      0 reactions
      		 Last Post SEQadmin2
      by SEQadmin2
      Yesterday, 05:37 AM  
      Large-Scale Protein Screen Uncovers Hidden Regulators of Alternative Polyadenylation by SEQadmin2
      Started by SEQadmin2, 06-26-2026, 11:10 AM
      0 responses
      17 views
      0 reactions
      		 Last Post SEQadmin2
      by SEQadmin2
      06-26-2026, 11:10 AM  
      Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population by SEQadmin2
      Started by SEQadmin2, 06-17-2026, 06:09 AM
      0 responses
      52 views
      0 reactions
      		 Last Post SEQadmin2
      by SEQadmin2
      06-17-2026, 06:09 AM  
      Sequencing the Two-Toed Sloth Genome Reveals Jumping Genes Tied to Its Extreme Metabolism by SEQadmin2
      Started by SEQadmin2, 06-09-2026, 11:58 AM
      0 responses
      110 views
      0 reactions
      		 Last Post SEQadmin2
      by SEQadmin2
      06-09-2026, 11:58 AM  
      View All
      Working...