Error while running clustalw2 in biopython

[bio_informatics]

import re, sys, argparse, os, subprocess
from subprocess import call
from Bio import SeqIO
from Bio import AlignIO
from Bio.Seq import Seq
from Bio.Align.Applications import ClustalwCommandline

for key in store_seq:
    fileN=str(key)+".txt" #"1.txt"
    call(["touch",fileN])
    outFile=str(key)+".fasta" #"1.fasta"
    
    with open(fileN,'w') as f:
        f.write(">"+str(store_seq[key])+"\n") # print seq id
        f.write(str(chemo_seq[store_seq[key]] )+"\n")#print sequence
        f.write( ">"+str(brig_orth[store_seq[key]])+ "\n")
        f.write ( str(chemo_seq[brig_orth[store_seq[key]]]) +"\n")
        f.write( ">"+str(reman_orth[store_seq[key]]) +"\n" )
        f.write( str(chemo_seq[reman_orth[store_seq[key]]]) + "\n")
         #run clustalw2 for the file..--->      
cline=ClustalwCommandline("clustalw2",infile=fileN,type="PROTEIN",output="FASTA",outfile=outFile,quiet)
    cline()

    #one key ends..

Error:cline=ClustalwCommandline("clustalw2",infile=fileN,type="PROTEIN",output="FASTA",outfile=outFile,quiet)
SyntaxError: non-keyword arg after keyword arg

In the above code, cline=Clus.. is not indenting with with open, kindly pardon me.

However, when I run on python shell it runs smoothly. I have clustaw2 in my path. Instead of fileN and outFile, I give file name in double quotes.

Editor using: emacs
What is the cause for this?
How do I correct it?
Python 2.7

[maubp]

The error is in your Python syntax, probably you need quiet=True at the end of the line defining cline.

Right now you have several keyword arguments (key=value) followed by a non-keyword argument (value). In Python any non-keyword arguments (also called positional arguments) must come before any keyword arguments.

[maubp]

This is a duplicate of https://www.biostars.org/p/107528/ - please don't double post like this without cross linking. It saves duplication of effort.

[bio_informatics]

Hi maubp,
Thank you for pointing that.
I didn't understand this point:

Right now you have several keyword arguments (key=value) followed by a non-keyword argument (value). In Python any non-keyword arguments (also called positional arguments) must come before any keyword arguments.

I apologize for multi posts.

[maubp]

Try this:

cline=ClustalwCommandline("clustalw2", infile=fileN, type="PROTEIN", output="FASTA", outfile=outFile, quiet=True)

[bio_informatics]

Thank you.

Try this:

cline=ClustalwCommandline("clustalw2", infile=fileN, type="PROTEIN", output="FASTA", outfile=outFile, quiet=True)