Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • gringer
    David Eccles (gringer)
    • May 2011
    • 845

    2-hour run on Oxford Nanopore MinION

    I am participating in the MinION access programme, and noticed that Nick Loman recorded a Nanopore run last night, using the most recent v7.3 flow cells. You can see the final read length histogram here:



    [or watch from the start to see the entire 2h run]

    This run produced a little under one read per second, with average read length about 7kbp, maximum sequence length of about 68kbp, producing about 45 Mbp over the course of the run. These numbers are estimated from the event data produced by the software, and the number of mappable bases will be less than this.

    To reduce confusion slightly, the histogram shown in the MinKNOW program here is adjusted so that the bin height displays the summed length of sequences in each bin, rather than the number of sequences. As an example of interpretation for this run, the most base pairs were generated from sequences that were about 20kbp long.

Latest Articles

Collapse

  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
30 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-09-2026, 11:58 AM
0 responses
44 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-05-2026, 10:09 AM
0 responses
51 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-04-2026, 08:59 AM
0 responses
51 views
0 reactions
Last Post SEQadmin2  
Working...