ATAC seq lysis time

[zoe561]

Hello,

I was wondering if anyone has attempted the ATACseq protocol on primary mouse basal keratinocytes and has some idea of optimal lysis time for 50,000 cells?

Thanks!

[Agun]

Hello, did you find the answer you needed?

I am also interested in ATAC-seq for our population of keratinocytes. Perhaps we can help eachother out.

[obischof]

Hello,

I was wondering if anyone has attempted the ATACseq protocol on primary mouse basal keratinocytes and has some idea of optimal lysis time for 50,000 cells?

Thanks!

Has any of you two tried ATACseq yet and if yes do you see a nucleosomal-like pattern after tagmantation? In our hands we only see a smear.

[Agun]

No, It seems a little bit complicated without having seen anybody do it.

What is your experience?