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Does anyone have a protocol for paired-end for Agilent's SureSelect array-based sequence capture?
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Do you have an issue with Agilent's protocol? -
The only Agilent protocol I have is for single-end and I'm interested in trying paired-end for the Illumina GA.Comment
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Version 1.0, September 2009
(top hit [.pdf])Comment
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Thanks but I'm specifically looking for paired-end for the array-based capture. While Agilent does have protocols for paired-end in-solution capture products (I've used those and they work quite well), Agilent only offers a protocol for single-end for the array-based capture product.Comment
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Oh. Any particular reason you're going with array based then, if solution based works so well?Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.Today, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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