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  • luc
    Senior Member
    • Dec 2010
    • 469

    #31
    But counting applications are much better served by the HiSeq - if one does not need immediate turnaround.


    Originally posted by M4TTN View Post
    Info from Illumina:

    The MiniSeq is similar to the technology utilised with our NextSeq platform based on 2-channel SBS chemistry.

    Pricing Information

    MiniSeq System (SY-420-1001): £35,653
    MiniSeq Basic Plan (20004132): £3,350 for 1 additional year’s cover
    MiniSeq Comprehensive Plan (20004133): £4,021 for 1 additional year’s cover

    Code:
    High Output Kit 	2 x 150 (300bp)	7.5	25	FC-420-1003	1,046
    High Output Kit 	2 x 75 (150 bp)	3.75	25	FC-420-1002	652
    High Output Kit 	1 x 75 (75 bp)	1.875	25	FC-420-1001	558
    Mid  Output Kit	2 x 150 (300 bp)	2.4	8	FC-420-1004	384
    So upfront and service costs are half a MiSeq.
    Output for counting experiments is the same as MiSeq v3 chemistry (25 M reads)
    The only thing it lacks are long paired reads 2x300 bp (but those kits are failing at the moment anyway...) and with NexteraXT many of our fragments are shorter than 300 bp anyway...

    I think knowing what I know now...I'd probably buy one...specs are only going to improve.

    Comment

    • M4TTN
      Member
      • Jan 2014
      • 77

      #32
      Depends on how many reads you need...we are multiplexing 5-8 samples quite nicely on a MiSeq (counting expt). No need to go to higher depth for us (at least not at the moment).

      Comment

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